Room 1670 Beyster Building
Title: Effect of storage on the microbiota and antibiotic resistance genes in source-separated urine (seminar announcement attached)
Speaker: Adey Desta, Ph.D.
Assistant Professor, Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia
Urine recycling is an ancient and modern practice that has positive implications for agriculture, environmental quality and energy efficiency as they pertain to present day wastewater treatment. Despite its benefits, urine has the potential to harbor pathogenic microorganisms and antibiotic resistance genes (ARGs) as a result of contamination by fecal matter and urinary tract infection. The current study is conducted to address the hypothesis that storage of source- separated urine increases its safety for agricultural use. The microbiome of fresh urine collected during two public events in summer 2014 and stored for two months were investigated using a shotgun metagenome sequencing approach. The results reveal the presence of 136 bacterial genera, five of which are pathogenic among the top ten most abundant genera in the samples including Streptococcus, Enterobacter and Tissierella. A total of 87 ARGs were identified with different frequencies in the samples from the two events. In both samples, storage was shown to be effective in reducing the aerobic pathogens and genes mediating resistance to Beta lactam, aminoglycoside and tetracycline antibiotics. Storage of source- separated urine can be a useful strategy for optimal elimination of most of the pathogens and ARGs from urine.